Molecular cloning steps in order include:

A、extractionofvectorDNA,ligationoftargetDNAtovectorDNA,restrictionoftargetDNA;
B、restrictionoftargetDNA,ligationofcut(thatis,restricted)targetDNAtovectorDNAwhichhasalreadybeencut,transformationofhostcells;
C、cuttingtargetDNA,cuttingvectorDNA,amplifyingtargetDNA;
D、amplifyingtargetDNA,ligatingtargetDNAtocutvectorDNA,homologousrecombinationoftargetandvectorDNA