Which of the following steps is required to manually obtain a hybrid plant? （ ）

A、Isolateprotoplasts→induceprotoplastfusion→culture→gethybridplants
B、Separationofprotoplasts→fusionofprotoplasts→hybridplants
C、Obtaincells→Protoplastfusion→Cultivation→Obtainhybridplants
D、Obtaincells→directfusionofprotoplasm→culture